OYINDASOLA OLUWABUKUNMI DEBORAH
Therapeutic Action of Ketogenic Diet on Cyclophosphamide-Induced Redox, Hepatic and Nephrotoxicity in Male Wistar Rats - Dr. O. T. Kayode - Mountain Top University BIOCHEMISTRY August,2023 - 90p.
Background: Strong chemotherapeutic drug cyclophosphamide (CP) is frequently linked to serious adverse effects that limit its therapeutic efficacy. The therapeutic potential of very low carbohydrate ketogenic diets (VLCKD) in several illness situations has attracted interest recently. Uncertainty persists regarding its impact on the hepatic enzymes and redox state in animals treated with CP. The goal of this study was to find out how a ketogenic diet (KD) may treat cyclophosphamide-induced redox imbalance, liver damage, and nephrotoxicity in a Wistar rat model. Methodology: Male Wistar rats (36) were divided into six (6) groups of six (6) animals each, Group 1 (Standard Diet), Group 2 (ketogenic feed), Group 3 (Standard diet and βHB (0.025mg/kg) was intubated), Group 4 (Standard Diet and intraperitoneally cyclophosphamide (200mg/ml)), Group 5 (ketogenic feed and interperitoneally cyclophosphamide (200mg/ml)), Group 6 (intraperitoneally cyclophosphamide and βHB (0.025mg/kg body weight) was
intubated) treatment lasted for five (5) weeks (35 days) after which the animals were sacrificed. Blood samples, liver, and kidney were collected for the analysis of the samples and the samples were subjected to ANOVA used for statistical analysis.Results: Serum levels of urea, creatinine, sodium, chloride and activity of MDA, AST, ALT, and ALP were significantly increase (p<0.05) in the CP-treated group compared to the control group indicating that CP caused considerable renal and hepatic damages. In addition, as compared to the control group, the CP-treated group had significantly decrease (p<0.05) kidney levels of
potassium, and the activity of SOD and catalase. Conclusion: The results highlight the potential of KD as a further therapeutic approach to reduce chemotherapy-related side effects and improve overall treatment outcomes. the KD greatly decreased the oxidative stress caused by CP. This was demonstrated by lower levels of reactive oxygen species (ROS) and lipid peroxidation in the tissues of the hepatic and renal organs.Keywords: Cyclophosphamide, Hepatic enzymes, Ketogenic Diet, Kidney indices
BIOCHEMISTRY
Therapeutic Action of Ketogenic Diet on Cyclophosphamide-Induced Redox, Hepatic and Nephrotoxicity in Male Wistar Rats - Dr. O. T. Kayode - Mountain Top University BIOCHEMISTRY August,2023 - 90p.
Background: Strong chemotherapeutic drug cyclophosphamide (CP) is frequently linked to serious adverse effects that limit its therapeutic efficacy. The therapeutic potential of very low carbohydrate ketogenic diets (VLCKD) in several illness situations has attracted interest recently. Uncertainty persists regarding its impact on the hepatic enzymes and redox state in animals treated with CP. The goal of this study was to find out how a ketogenic diet (KD) may treat cyclophosphamide-induced redox imbalance, liver damage, and nephrotoxicity in a Wistar rat model. Methodology: Male Wistar rats (36) were divided into six (6) groups of six (6) animals each, Group 1 (Standard Diet), Group 2 (ketogenic feed), Group 3 (Standard diet and βHB (0.025mg/kg) was intubated), Group 4 (Standard Diet and intraperitoneally cyclophosphamide (200mg/ml)), Group 5 (ketogenic feed and interperitoneally cyclophosphamide (200mg/ml)), Group 6 (intraperitoneally cyclophosphamide and βHB (0.025mg/kg body weight) was
intubated) treatment lasted for five (5) weeks (35 days) after which the animals were sacrificed. Blood samples, liver, and kidney were collected for the analysis of the samples and the samples were subjected to ANOVA used for statistical analysis.Results: Serum levels of urea, creatinine, sodium, chloride and activity of MDA, AST, ALT, and ALP were significantly increase (p<0.05) in the CP-treated group compared to the control group indicating that CP caused considerable renal and hepatic damages. In addition, as compared to the control group, the CP-treated group had significantly decrease (p<0.05) kidney levels of
potassium, and the activity of SOD and catalase. Conclusion: The results highlight the potential of KD as a further therapeutic approach to reduce chemotherapy-related side effects and improve overall treatment outcomes. the KD greatly decreased the oxidative stress caused by CP. This was demonstrated by lower levels of reactive oxygen species (ROS) and lipid peroxidation in the tissues of the hepatic and renal organs.Keywords: Cyclophosphamide, Hepatic enzymes, Ketogenic Diet, Kidney indices
BIOCHEMISTRY