| Summary, etc |
Polycystic Ovarian Syndrome (PCOS) or Stein- Leventhal syndrome is a metabolic endocrine<br/>disorder that is very common in women of reproductive age affecting approximately 2-10% of<br/>them. Although several synthetic drugs such as metformin are available for the treatment of PCOS,<br/>the side effects of the use of metformin such as lactic acidosis have continued to limit their<br/>acceptability. Thus, an alternative option of plants like Ocimum gratissimum leaves should be<br/>investigated on. The biochemical and toxicological effects of ethanolic extract of Ocimum<br/>gratissimum leaves (EEOGL) at the doses of 50 and 100mg/kg body weight on letrozole-induced<br/>in Polycystic Ovarian Syndrome (PCOS) was investigated in female Wistar rats. Twenty female<br/>Wistar rats with an average weight of (170.81±5.25g) were assigned into 5 groups (A-E) of four<br/>each: animals in group A were the control group which received rat pellets and water for 30days<br/>while the letrozole-induced rats in groups B, C, D and E also received distilled water, 7.14mg/kg<br/>body weight of metformin and 2mg/kg body weight of clomiphene citrate (reference drugs) and<br/>the same volume of the extract corresponding to 50 and 100mg/kg body weight of EEOGL<br/>respectively after which levels of some biochemical and toxicological indices were determined<br/>after each rats were sacrificed by assessing liver function indices, kidney function indices, AST,<br/>ALP, ALT data were subjected to analysis of variance and Duncan multiple Range test with<br/>statistical significance set at p<0.05. The result reveals: Nine different secondary metabolites were<br/>present in the EEOGL, the highest Phyto-components present in the EEOGL is Ethanone, 1-(6,6-<br/>dimethylbicyclo [3.1.0] hex-2-en-2-yl)- with the retention time of 11.592 and the lowest is<br/>Benzene, 4-ethenyl-1,2-dimethyl- with the retention time of 0.01, a significant increase (p<0.05)<br/>and significant decrease (p<0.05) at dose of 50mg/kg and 100mg/kg of EEOGL on kidney<br/>function indices as well as liver function indices was noticed. Therefore, the administration of<br/>EEOGL at 50 and 100 mg/kg B.W. to letrozole-induced PCOS rats shows mild alteration in the<br/>assayed biochemical indices. |
