| Summary, etc |
Polycystic ovarian syndrome (PCOS) is a multi-symptom endocrine disorder that results from androgen excess, particularly testosterone, and ovarian dysfunction that disrupts HPO axis function. Women with PCOS possess an altered immune response system which presents a low-grade chronic inflammatory state in them. Serum cytokine levels in the letrozole-induced PCOS rats will be investigated after treatment with Alpha lipoic acid and Olive oil and the results compared with a healthy control group. Thirty-five rats were used for this experiment,<br/>each group consisting of seven rats. Group 1 was the control group, while Groups 2 – 5 were induced with 1ml/1kg of letrozole for 22 days. Drug administration for the rats distributed into five groups are as follows: the non-PCOS control group was administered 0.5ml of saline water, the PCOS control group was administered 0.5ml of distilled water, and three PCOSinduced groups were administered 2mg/kg of clomiphene citrate and 7.14mg/kg of metformin, 1mg/kg of alpha lipoic acid and 4ml/kg of olive oil respectively for 14 days. At<br/>the end of the experimental period, the rats were anaesthetized with diethyl ether and sacrificed by jugular puncture 25 hours after the last treatment. The serum Tumor Necrosis Factor (TNF-𝛼) and Interleukin (IL-6) levels of letrozole-induced polycystic ovarian syndrome rats were assayed for and the results were analyzed. It was reported in this study, that letrozole-induced PCOS Wistar rats possess high serum levels of TNF- 𝛼 and IL-6. Alpha lipoic acid and Olive oil exert anti-inflammatory potentials against IL-6 released into the bloodstream compared favourably with metformin and clomiphene citrate. Olive oil showed a great anti-inflammatory potential against TNF- 𝛼 release into the bloodstream. Therefore, it can be exploited as a management option in the treatment of PCOS. |
