Mountain Top University Library

ABSTRACT
Eugenia caryophyllata (clove) was extracted by cold extract using deionised water and characterized to determine the phytochemicals and structures of the extract using qualitative study, GC-MS and NMR analysis. The results showed the presence of phytochemicals except Protein- Amino acids which is absent. The phytochemicals evaluated in the aqueous extract of Eugenia caryophyllata (clove) were in the order of total phenolics > flavonoids with the respective values of 6.6 > 2.6 mg/g of extract. Presence of high level of phenolic compounds revealed that Eugenia caryophyllata (clove) extract can have good antioxidant capacity. The resulting spectrum of ¹H NMR analysis found that the existence of distinct peaks signals at 2.36, intensity of 4134.6 and 4011.2 in the range of 2.47 – 2.25 with reference to the proton of acids H-C-OOH, carbonyl compounds HC–C=O, aromatic methyl group Ar–H (Ar–C–H, benzylic ) and Methyl ketone group HC– (R)-C=O. The results demonstrate the characterisation of Eugenia caryophyllata (clove) revealing the phytochemical properties and isolated compounds in relation to its general use as a medicinal plant.
Keywords: Eugenia caryophyllata (clove); Erythrocyte Osmotic Fragility Test, GC-MS and NMR.