Screening and Production of Lipase from Lipolytic Fungi Under Submerged Fermentation
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TextPublisher: Ibafo Department of Biological Sciences 2020Edition: Mr. G.E. AdebamiDescription: xi,; 34p. tablesSubject(s): Natural Science -- BiologySummary: Lipases are triacylglycerol acyl hydrolases with the enzyme number EC3.1.1.3. They catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. In this styudy, fungi were isolated from diesel and restaurant wasrewater polluted soil and screened for lipase production on solid agar. The morphorlogical and biochemical characteristics of the best isolated were determined. The effects of carbon and Nitrogen sources on Lipase production were also investigated. A total of 11 fungi were isolated. The diameter of growth ranged from 24.6i-47.0a mm and lipase production ranged from 0.0h mm-10.5a mm. Isolated VPD4 had the best lipase activity and was identified as fusarium oxysporum. Carbon sources had significant difference (p>0.05)
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in lipase production which ranged from 79.92g U/mL- 182.22a U/mL U/mL- 182.22a U/mL respectively. Glucose (188.65a U/mL) and peptone (182.22a U/mL) supported the highest lipase production. This study has shown that futsarium oxysporum isolated from a diesel polluted soil is a good lipase producer and can be further investigated for industrial purpose.
Lipases are triacylglycerol acyl hydrolases with the enzyme number EC3.1.1.3. They catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. In this styudy, fungi were isolated from diesel and restaurant wasrewater polluted soil and screened for lipase production on solid agar. The morphorlogical and biochemical characteristics of the best isolated were determined. The effects of carbon and Nitrogen sources on Lipase production were also investigated. A total of 11 fungi were isolated. The diameter of growth ranged from 24.6i-47.0a mm and lipase production ranged from 0.0h mm-10.5a mm. Isolated VPD4 had the best lipase activity and was identified as fusarium oxysporum. Carbon sources had significant difference (p>0.05)
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in lipase production which ranged from 79.92g U/mL- 182.22a U/mL U/mL- 182.22a U/mL respectively. Glucose (188.65a U/mL) and peptone (182.22a U/mL) supported the highest lipase production. This study has shown that futsarium oxysporum isolated from a diesel polluted soil is a good lipase producer and can be further investigated for industrial purpose.
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