Mountain Top University Library

Salmonella is mostly common in domestic and wild animal and pose a serious threat to the civilization because of their ability to adapt to their surroundings which has a great impact on their genetic makeup. Plasmid is a significant topic to research since it is crucial to antibiotic resistance.This study is evaluated to determine the antibiotics susceptibility pattern and the presence of
detectable plasmid of Salmonella isolate obtained from food animals to better understand their antibiotic resistance and toxicity. Antibiotic susceptibility testing was done to determine the association between plasmid presence and antibiotic resistance patterns. Plasmid profiling was done using plasmid DNA extraction kit and agarose gel electrophoresis while curing was done using Sodium dodecyl sulphate. The result shows that there were various plasmids differing in size and number among the Salmonella isolate. However, among the 15 Salmonella isolate only 5 Salmonella isolate were cured. After curing, resistance of the isolates to the antibiotics were not plasmid-mediated but could be chromosomally mediated or maybe due to the presence of efflux
pumps. This research demonstrated the presence of Salmonella in food animals are the major source of antibiotic-resistant Salmonella and has a potential as a useful tool for comprehending the dynamics of Salmonella isolates from food animals.
Keyword: Salmonella, Food animal, plasmid, plasmid profiling, plasmid curing, Antibiotic resistant, Antibiotic susceptibility testing and SDS