Mountain Top University Library

Staphylococcus aureus, a Gram-positive coccus has been recognized to be an important pathogen both in the Community and in the Hospital. The aim of this study was to determine the prevalence of Staphylococcus aureus on railings and to determine the antimicrobial profile of the isolates recovered to various antibiotics. A total number of 33 samples were obtained from railings in Boys’ hostel (New Daniel Hall) in Mountain Top University. These samples were collected from both A wing and B wing alongside some other rails (River bridge, R, college underground, CU, college floor three, C3) of the University environment. The samples were analyzed using standard conventional methods and materials in order to isolate, identify and carry out the antimicrobial
susceptibility testing. Subsequent Culturing using nutrient broth was carried out. Nutrient agar, Mannitol Salt agar was used for the isolation process. Biochemical test such as Catalase test, Coagulase tests, Antibiotic Sensitivity test were carried out to identify the isolates. Strict Sterilization process was ensured while working on the cultures and the materials used. The antibiotic susceptibility test was conducted using Kirby and Bauer disc diffusion method and the results was determined using the clinical and laboratory standard institute (CLSI) 2016 guide. The total number of 33 (94%) Staphylococcal isolates were recovered from 35 samples when streaked on Mannitol salt agar (MSA). And 22 (66%) isolates were confirmed to be Staphylococcus aureus, while 9 were confirmed to be Methicillin resistant. The highest rate of isolation of Staphylococcus aureus isolates were obtained from samples collected from A wing Boys’ hostel, followed by samples collected from others. 100% of the Staphylococcus aureus isolates were susceptible to Streptomycin (68%), Ciprofloxacin (5%), Rocephin (23%), Amoxacillin (13%), Zinnacef (13%), Ampiclox (9%), Gentamicin (63%), Pefloxacin (13%), Erythromycin (5%), Sulfamethoxazole (50%). Findings from this study demonstrate that Staphylococcus aureus, which is capable of infecting people, is present on the railings of Boy’s hostel at Mountain Top University. Serious health issues may result from these microbes. Therefore, maintaining excellent personal hygiene practices like washing your hands frequently will help to lower the risk of microbial transmission.