EVALUATION OF THE EFFECT OF Amaranthus hybridus ON LIPID PROFILE IN SOFT DRINK (BIGI COLA)-FED Drosophila melanogaster
- PROF.G.O. AJAY
- Mountain Top University BIOCHEMISTRY August,2023.
- 76p.
Soft drinks like cola drinks contain caffeine which could create oxidative imbalance in system that can lead to generation of reactive oxygen species (ROS) which are highly reactive with molecules such as lipids, DNA, proteins and other macromolecules. Amaranthus hybridus, a common vegetable plant is noted to possess phytochemicals with antioxidative properties which could mitigate the effect of the ROS on lipids and others. Therefore, this study is design to evaluate the effect of A. hybridus on lipid profile in Bigi colainduced Drosophila melanogaster. Three hundred (300) Fruit flies (Drosophila melanogaster) were obtained and distributed into 5 groups of 20 flies in triplicates. Group 1 is control, group 2 received Bigi Cola only (0.5 μl), group 3 received Bigi Cola (0.5 μl) and Amaranthus hybridus (0.5 μl), group 4 received Bigi Cola (0.5 μl) and Vitamin C (50 mg/ml) and group 5 received Amaranthus hybridus only (0.5 μl) for 10 days. Thereafter, flies were put to sleep on ice, homogenized in phosphate buffer saline (0.1 M, pH 7.4), centrifuged, supernatant collected and stored in the freezer at -4℃ until used for lipid analysis. The results showed that administration of Bigi cola significantly increased the concentrations of triglyceride, HDL, VLDL and LDL and reduced the concentration of total cholesterol compared to control. Whereas, treatment with A. hybridus further slightly increased the concentrations of triglyceride and VLDL but reversed the trend in the concentrations of total cholesterol, HDL and LDL compared to the Bigi cola group. In conclusion, the results suggest that A. hybridus has the potenial to lower the hyperlipidemic effect of Bigi cola in D. melanogaster. KEYWORDS: Drosophila melanogaster, Reactive Oxidative Species(ROS), Amaranthus hybridus, hyperlipidemia, homogenized.