000			01831nam a22001457a 4500
008			211021b ||||| |||| 00| 0 eng d
100			_aAKINDELE, Blessing Oluwadamilola. _98980
245			_aSCREENING, PRODUCTION AND CHARACTERIZATION OF LIPASE FROM Saccharomyces cerevisiae
250			_aDr. G.E. Adebami
260			_aIbafo _bMicrobiology _c2021
300			_axiii;63p.
520			_aABSTRACT Lipases (E.C. 3.1.1.3) are hydrolytic enzymes with biotechnological potentials. They play important roles in the hydrolysis of triglycerides to free fatty acids and glycerol as commercial biocatalysts. In this study, lipase producing yeast isolated from palm wine and fermented fruits were screened by assessing various biochemical and physico-chemical parameters. The best isolate was identified, and it's morphological and biochemical properties were evaluated. The effect of different carbon sources, nitrogen sources, temperature and pH were investigated on the lipase production by the isolate. A total of 11 yeast species were isolated and screened for lipase production. Isolate BPWP2 produced the highest lipase activity and was identified as Saccharomyces cerevisiae. Carbon sources, nitrogen sources, incubation temperature and initial pH had significant effects (p<0.05) on lipase production. Glucose (75.55a U/mL), yeast extract (88.05a U/mL), 30℃ (79.50a U/mL) and pH 6.0 (85.63a U/mL) supported the optimum lipase production by the selected isolate. In conclusion, the results of this study suggest conditions to ensure a high yield in the production of lipase enzyme using Saccharomyces cerevisiae isolated from the fermented fruits and palm wine. Key words: Lipase production, carbon source, nitrogen source, temperature, Saccharomyces cerevisiae
650			_aNatural Science _vMicrobiology _98979
942			_cTHS
999			_c6474 _d6474