000			01799nam a22001457a 4500
008			211021b ||||| |||| 00| 0 eng d
100			_aABBA, Praise Oluwatobiloba. _98981
245			_aSCREENING AND PRODUCTION OF LIPASE FROM Streptomyces sp.
250			_aDr. Adebami G.E.
260			_aIbafo _bMicrobiology _c2021
300			_axii;65p.
520			_aABSTRACT Lipases (EC 3.1.1.3) is a triacylglycerol acylhydrolase that works on carboxylic ester linkages. They belong to the class of serine hydrolases and do not require any cofactor. In this study, bacterial isolates from domestic wastewater contaminated soils were screened on solid agar for lipase production. The best isolate was identified using morphological and biochemical characterizations. Effects of physico-chemical parameters such as carbon and nitrogen sources, temperature, and pH on lipase production were investigated. A total of 15 bacterial species were isolated and screened for lipase production. Isolate PSN07 showed the highest lipase activity and was identified as Streptomyces sp. Carbon sources, nitrogen sources, incubation temperature and initial pH had significant effects (p<0.05) on lipase production. Glucose (65.95a U/mL), yeast extract (88.99a U/mL), 30oC (69.41a U/mL) and pH 6.0 (80.64a U/mL) supported the optimum lipase production by the selected isolate. Streptomyces sp. PSN07 isolated from domestic wastewater contaminated soil was a good lipase producer which can be developed for the industrial production of biotechnologically important products such as food, beverages, leather, textiles, detergents, and soaps. Keywords: Lipase production, carbon source, nitrogen source, temperature, Streptomyces sp.
650			_aNatural Science _vMicrobiology _98979
942			_cTHS
999			_c6475 _d6475