000 02010nam a22001457a 4500
008 230425b ||||| |||| 00| 0 eng d
100 _aADEBAYO Regina Mayowa
_99899
245 _aPREVALENCE OF Shigella IN READY TO EAT FRESH PRODUCE AND DRIED GAME MEAT
250 _aDR G.B Akann
260 _aMountain Top University
_bBiological Science
_c2022
300 _ax;43p
520 _aThe prevalence of shigella species in ready-to-eat (RTE) fresh produce and dried bush meat in Ogun state Nigeria was determined. 30 samples of fruits and vegetables consisting of Cabbage (Brassica oleracea var. capitate), carrots (Daucus carota subsp. Sativus), Pineapple (Ananas cosmosus), Watermelon (Citrullus lanatus), Cucumber (Cucumis sativus), Lettuce (Lactuca sativa) were bought from three different stalls in Magboro, market Ogun state.55 dried bush meat samples was also screened for the presence of shigella consisting of Antelope (Bovidae), Guinea Fowl (Numidae), Alligator, Hedgehog (Erinaceinae), Wild Rabbit (orytolagus cuniculus), Grasscutter (Thryonomys swinderianus), Pangolin (pholidota), Sparrow(passeridae) Bush dog (lyacon pictus) , Monkey (cercopithecidae) , Porcupine( erithizon dorsatum), Hare (lepus)and Quail (corturnix cortunix). Shigella species were found in 16 (53.3%) of the fresh produce samples. While in dried bush meat shigella was present in 25 (45.45%) The highest quantity of Shigella was found in lettuce, cabbage, and carrot, indicating that the fresh produce had been heavily contaminated by pathogenic bacteria. PCR was used to validate the shigella species using 16sRNA. The fresh produce and dried bush meat samples examined in this study were severely contaminated with shigella species, which could be attributed to poor food handler cleanliness. The general people should be made aware of the safety and hygiene of fresh produce and bush meat from these sites. Keywords: Shigella species, , Fresh produce, Bush meat, Ready-to-eat, PCR.
650 _aMicrobiology
942 _cTHS
999 _c7218
_d7218