| Summary, etc |
ABSTRACT<br/>Lipases (EC 3.1.1.3) is a triacylglycerol acylhydrolase that works on carboxylic ester linkages. <br/>They belong to the class of serine hydrolases and do not require any cofactor. In this study, <br/>bacterial isolates from domestic wastewater contaminated soils were screened on solid agar for <br/>lipase production. The best isolate was identified using morphological and biochemical <br/>characterizations. Effects of physico-chemical parameters such as carbon and nitrogen sources, <br/>temperature, and pH on lipase production were investigated. A total of 15 bacterial species were <br/>isolated and screened for lipase production. Isolate PSN07 showed the highest lipase activity and <br/>was identified as Streptomyces sp. Carbon sources, nitrogen sources, incubation temperature and <br/>initial pH had significant effects (p<0.05) on lipase production. Glucose (65.95a U/mL), yeast <br/>extract (88.99a U/mL), 30oC (69.41a U/mL) and pH 6.0 (80.64a U/mL) supported the optimum <br/>lipase production by the selected isolate. Streptomyces sp. PSN07 isolated from domestic <br/>wastewater contaminated soil was a good lipase producer which can be developed for the <br/>industrial production of biotechnologically important products such as food, beverages, leather, <br/>textiles, detergents, and soaps. <br/>Keywords: Lipase production, carbon source, nitrogen source, temperature, Streptomyces sp.<br/> |
