Mountain Top University Library

ABSTRACT
Lipases (EC 3.1.1.3) is a triacylglycerol acylhydrolase that works on carboxylic ester linkages.
They belong to the class of serine hydrolases and do not require any cofactor. In this study,
bacterial isolates from domestic wastewater contaminated soils were screened on solid agar for
lipase production. The best isolate was identified using morphological and biochemical
characterizations. Effects of physico-chemical parameters such as carbon and nitrogen sources,
temperature, and pH on lipase production were investigated. A total of 15 bacterial species were
isolated and screened for lipase production. Isolate PSN07 showed the highest lipase activity and
was identified as Streptomyces sp. Carbon sources, nitrogen sources, incubation temperature and
initial pH had significant effects (p<0.05) on lipase production. Glucose (65.95a U/mL), yeast
extract (88.99a U/mL), 30oC (69.41a U/mL) and pH 6.0 (80.64a U/mL) supported the optimum
lipase production by the selected isolate. Streptomyces sp. PSN07 isolated from domestic
wastewater contaminated soil was a good lipase producer which can be developed for the
industrial production of biotechnologically important products such as food, beverages, leather,
textiles, detergents, and soaps.
Keywords: Lipase production, carbon source, nitrogen source, temperature, Streptomyces sp.